6533b7dcfe1ef96bd12721de

RESEARCH PRODUCT

Relaxation and cyclic GMP levels in response to sildenafil in human pulmonary arteries from donors.

Martin AldasoroGloria SegarraJuan Martínez-leónMaría Dolores MauricioPascual MedinaJosé M. Vila

subject

AdultMaleNitroprussideEndotheliumSildenafilPhosphodiesterase InhibitorsVasodilator AgentsVasodilationPharmacologyIn Vitro TechniquesPulmonary ArteryPiperazinesSildenafil CitrateNitric oxidechemistry.chemical_compound3'5'-Cyclic-GMP PhosphodiesterasesmedicineHumansNitric Oxide DonorsSulfonesCyclic GMPPharmacologybiologyChemistryDrug SynergismMiddle Agedrespiratory tract diseasesNitric oxide synthaseVasodilationmedicine.anatomical_structureBiochemistryEnzyme inhibitorPurinesCirculatory systemcardiovascular systembiology.proteinFemaleSodium nitroprussidemedicine.drug

description

We measured cyclic GMP formation and relaxation response to sildenafil given either alone or in combination with sodium nitroprusside (SNP) in pulmonary arteries obtained from 13 multi-organ donors. Sildenafil (10(-9)-10(-4) M) caused concentration-dependent relaxations and amplified the relaxation induced by SNP. Relaxation was unaffected by endothelium removal or by pre-treatment with the inhibitor of nitric oxide synthase L-NMMA (10(-4) M). SNP (10(-7) M) caused elevation of cyclic GMP levels that was potentiated by sildenafil (10(-6) M). Thus, the enhancement of SNP-induced relaxation by sildenafil is mainly due to an increase in cyclic GMP accumulation.

yearjournalcountryeditionlanguage
2005-10-18European journal of pharmacology
10.1016/j.ejphar.2005.11.042https://pubmed.ncbi.nlm.nih.gov/16380114