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RESEARCH PRODUCT
Effects of thrombin and of the phospholipase C inhibitor, D609, on the vascularity of the chick chorioallantoic membrane.
Christiana DimitropoulouMichael E. MaragoudakisM. A. Konerdingsubject
Bridged-Ring Compoundsmedicine.medical_specialtyanimal structuresAngiogenesisPhosphodiesterase InhibitorsNeovascularization PhysiologicChick EmbryoBiologyHemostaticsMicrocirculationThrombinVascularityAllantoisThiocarbamatesInternal medicinemedicineAnimalsOvumPharmacologyBlood VolumePhospholipase CThrombinThionesChorionNorbornanesChorioallantoic membraneEndocrinologymedicine.anatomical_structureType C PhospholipasesCirculatory systemMicroscopy Electron Scanningmedicine.symptommedicine.drugBlood vesseldescription
Abstract Microvascular corrosion casting was used to assess the effects of thrombin and D609, a phospholipase C inhibitor, on the vascularity of the chick embryo chorioallantoic membrane (CAM). Discs containing vehicle, thrombin or D609 were placed on the CAM of fertilized white Leghorn eggs on Day 9 of gestation and vascularity was assessed on Day 11. Thrombin caused significant increases in the numbers (43%), diameters (5%) and lengths (17%), of both pre- and postcapillaries (first-order vessels by centripetal ordering). Conversely, D609 caused a decrease in the numbers (27%), lengths (12%) and diameters (8%) of first-order vessels. D609 decreased the total vascular volume of first- to third-order vessels by 32%, whereas thrombin increased vascular volume by 27%. Additionally, thrombin increased capillary plexus density by 6%, whereas D609 decreased capillary plexus density by 3%. These findings provide a quantitative assessment of changing vascularity in the chick CAM—a model assay system in the development of pro- and antiangiogenic agents.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-11-01 | General pharmacology |