Crypthecodinium cohnii metabolisma matemātiskā modelēšana DHA ražošanas optimizācijai

6533b7dcfe1ef96bd1272e56

RESEARCH PRODUCT

Crypthecodinium cohnii metabolisma matemātiskā modelēšana DHA ražošanas optimizācijai

subject

kinētiskā modelēšana Dokozaheksaēnskābe Crypthecodinium cohnii stehiometriskā modelēšana Bioloģija

description

Datormodelēšana un bioķīmisko ceļu simulēšana ir sistēmbioloģijas pamatnostādnes, un pastāv vairākas metodes, kas var palīdzēt šo sistēmu izpētīšanā. Darbā izmantota divu sistēmbioloģijas modelēšanas metožu pieeja šūnu taukskābju sintēzes metabolisma pētīšanai. Darba ietvaros izveidots kinētiskais modelis, kas ietver glikolīzes ceļa reakcijas un reakcijas, kas ved līdz taukskābju sintezējošajam enzīmam- FAS, kā arī pilnveidots genoma izmēra metaboliskais modelis dinoflagellātam Crypthecodinium cohnii. Kinētiskais modelis tika parametrizēts saskaņā ar pieejamajiem eksperimentālajiem datiem. Aplūkoti stehiometriskajā modelī ietvertie metabolisma regulācijas mehānismi taukskābju sintēzei un galvenā prekursorā metabolīta iesaiste organisma reakcijās. Turpmāk abus modeļus paredzēts izmantot stratēģiju veidošanā pētāmā organisma metabolisma optimizācijai. Mērķis ir palielināt dokozaheksaēnskābes ražošanu, un veikt pareizo substrātu izvēli organisma audzēšanai bioreaktorā.

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/50698

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