6533b7dcfe1ef96bd127330e
RESEARCH PRODUCT
Intravital fluorescence microscopy for the study of leukocyte interaction with platelets and endothelial cells
Brigitte VollmarHans-anton LehrMichael D. MengerPeter Vajkoczysubject
chemistry.chemical_classificationReactive oxygen speciesKupffer cellCellBiologymedicine.disease_causeMicrocirculationCell biologymedicine.anatomical_structurechemistryFluorescence microscopeHepatic stellate cellmedicineIntravital microscopyOxidative stressdescription
Publisher Summary This chapter introduces the techniques of intravital microscopy as a tool to study the microcirculation in intact animals under conditions of oxidative stress. Intravital fluorescence microscopy can be performed with almost all types of epiillumination microscopes available. The introduction of fluorescent dyes and refined epiillumination techniques has significantly advanced the possibilities of intravital microscopic studies of the liver microcirculation, including quantitative analyses of both circulatory parameters and cellular mechanisms. Using appropriate dyes, intravital microscopy allows the study of (1) microvascular perfusion, (2) leukocyte-endothelial cell interaction, (3) Kupffer cell and Ito cell function, and (4) hepatocellular membrane damage and mitochondrial redox state. The chapter discusses the role of reactive oxygen species (ROS) in hepatic microcirculatory dysfunction.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 |