6533b7dcfe1ef96bd127346d
RESEARCH PRODUCT
Phylogenetic reconstruction of the yeast genus Kluyveromyces: restriction map analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers
Carmela BellochM. Dolores GarcíaEladio BarrioAmparo Querolsubject
Electrophoresis Agar GelGeneticsPhylogenetic treebiologyRestriction MappingRibosomal RNAbiology.organism_classificationDNA RibosomalPolymerase Chain ReactionApplied Microbiology and BiotechnologyMicrobiology18S ribosomal RNARNA Ribosomal 5.8SKluyveromycesRestriction siteRestriction mapPhylogeneticsKluyveromycesRibosomal DNAPhylogenyEcology Evolution Behavior and SystematicsDNA Primersdescription
Summary We have constructed restriction site maps of the 5.8S rRNA gene and the two ITS regions in 60 strains of Kluyveromyces genus. We test the value of this region as a phylogenetic indicator, and its possible use as a fast and easy method to identify species of this genus. Despite some minor incongruences, our results are in good agreement with previous phylogenetic reconstructions based on the 18S rRNA gene sequencing (Cai et al., 1996; James et al., 1997). A highly significant monophyletic group was formed by K. lactis, K. marxianus, K. aestuarii, K. dobzhanskii and K. wickerhamii, which should be considered the true Kluyveromyces genus. The other species of the genus were grouped with lower bootstrap levels. Finally, the restriction map showed by three K. lactis strains, previously identified as K. marxianus var. drosophilarum, could be interpreted as indicatory of the possible existence of different species.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-08-15 |