6533b7dcfe1ef96bd12734a4
RESEARCH PRODUCT
Molecular cloning and characterization of the cDNA encoding the rat liver gamma-butyrobetaine hydroxylase
Jean DemarquoyPierre ClouetStéphane GallandBéatrice GeorgesGrand-jean FBouchard FLe Borgne Fsubject
MaleDNA Complementarygamma-Butyrobetaine DioxygenaseMolecular Sequence DataBiologyMolecular cloningMixed Function Oxygenaseschemistry.chemical_compoundSequence Homology Nucleic AcidComplementary DNAmedicineAnimalsAmino Acid SequenceCarnitineCloning MolecularRats WistarMolecular Biologychemistry.chemical_classificationMessenger RNAMethionineBase SequenceSequence Homology Amino AcidGene Expression Regulation DevelopmentalCell BiologyMolecular biologyRatsAmino acidOpen reading frameLiverchemistryBiochemistryCarnitine biosynthesisSequence Alignmentmedicine.drugdescription
Carnitine biosynthesis from lysine and methionine involves five enzymatic reactions. gamma-butyrobetaine hydroxylase (BBH; EC 1.14. 11.1) is the last enzyme of this pathway. It catalyzes the reaction of hydroxylation of gamma-butyrobetaine to carnitine. The cDNA encoding this enzyme has been isolated and characterized. The cDNA contained an open reading frame of 1161 bp encoding a protein of 387 amino acids with a deduced molecular weight of 44.5 kDa. The sequence of the cDNA showed an important homology with the human cDNA recently isolated. Northern analysis showed gamma-butyrobetaine hydroxylase expression in the liver and in some extend in the testis and the epididymis. During this study, it also appeared that BBH mRNA expression was undetectable by Northern analysis during the perinatal period. During the development of the rat, the amount of BBH mRNA appeared after the weaning of the young rat and reached a maximal expression at the adult stage.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-10-20 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids |