Effect of chronic citalopram on serotonin-related and stress-regulated genes in the dorsal raphe nucleus of the rat

6533b7ddfe1ef96bd1273671

RESEARCH PRODUCT

Effect of chronic citalopram on serotonin-related and stress-regulated genes in the dorsal raphe nucleus of the rat

Ursula Havemann-reinecke Gabriele Flügge Gabriele Flügge Nashat Abumaria Christoph Hiemke Eckart Rüther Eberhard Fuchs Eberhard Fuchs Rafal Rygula Rafal Rygula

subject

Male Serotonin endocrine system medicine.medical_specialty Citalopram Tryptophan Hydroxylase Biology Citalopram Polymerase Chain Reaction behavioral disciplines and activities Gene Expression Regulation Enzymologic 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Dorsal raphe nucleus Stress Physiological Internal medicine mental disorders medicine Animals Pharmacology (medical)Rats Wistar Social Behavior Neurotransmitter Biological Psychiatry Serotonin transporter 030304 developmental biology Pharmacology 0303 health sciences Tryptophan hydroxylase Rats Psychiatry and Mental health Endocrinology Neurology chemistry Models Animal biology.protein Synaptic vesicle glycoprotein 2B RNA Raphe Nuclei 5-HT1A receptor Neurology (clinical)Serotonin Selective Serotonin Reuptake Inhibitors 030217 neurology & neurosurgery medicine.drug

description

Using a model of depression in which chronic social stress induces depressive-like symptoms, we investigated effects of the selective serotonin-reuptake inhibitor (SSRI) citalopram on gene expression in the dorsal raphe nucleus of male rats. Expression of tryptophan hydroxylase (TPH) protein was found to be upregulated by the stress and normalized by citalopram, while mRNAs for genes TPH 1 and 2 were differentially affected. Citalopram had no effect on serotonin transporter mRNA but reduced serotonin-1A autoreceptor mRNA in stressed animals. The SSRI prevented the stress-induced upregulation of mRNA for CREB binding protein, synaptic vesicle glycoprotein 2b and the glial N-myc downstream-regulated gene 2, but increased mRNA for neuron-specific enolase (NSE) in both stressed and unstressed animals having no effect on stress-induced upregulation of NSE protein. These findings demonstrate that in the dorsal raphe nucleus of chronically stressed rats, citalopram normalizes TPH expression and blocks stress effects on distinct genes related to neurotransmitter release and neuroplasticity.

year	journal	country	edition	language
2007-05-01	European Neuropsychopharmacology

https://doi.org/10.1016/j.euroneuro.2006.08.009