6533b7ddfe1ef96bd1273e41

RESEARCH PRODUCT

Neuronal cell cultures: A tool for investigations in developmental neurobiology

I. Di LiegroGiovanni SavettieriA. CestelliGiuseppe Salemi

subject

Cell Survivalhormone supplemented-serum free-mediaBiologyBiochemistryExtracellular matrixCellular and Molecular NeuroscienceNeurobiologyNeurotrophic factorsSettore BIO/10 - BiochimicamedicineAnimalsHumansGrowth SubstancesDevelopmental neurobiologybookCells CulturedNeuronsNeuroscience (all)Cell DifferentiationGeneral MedicineCulture Mediamedicine.anatomical_structureCell cultureNeuronal cell culturebook.journalSettore MED/26 - NeurologiaNeuronNeuroscience

description

The aim of this review is to describe environmental requirements for survival of neuronal cells in culture, and secondly to survey the complex interplay between hormones, neurotrophic factors, transport- and extracellular matrix- proteins, which characterize the developmental program of differentiating neurons. An overall reconsideration of the literature in this vast field is above the limits of the present paper; since progress and refinement in the techniques of neuronal cell cultures have paralleled the advancement in Developmental Neurobiology, we will run instead through the main steps which form the conceptual framework of neuronal cell cultures. © 1992 Plenum Publishing Corporation.

https://doi.org/10.1007/bf00968395