6533b7ddfe1ef96bd1273e9d
RESEARCH PRODUCT
On-line gradient liquid chromatography–Fourier transform infrared spectrometry determination of sugars in beverages using chemometric background correction
Salvador GarriguesGuillermo QuintásM. De La GuardiaJulia Kuligowskisubject
ChemometricsMatrix (chemical analysis)Detection limitColumn chromatographyChromatographyResolution (mass spectrometry)ChemistryAnalytical chemistryInfrared spectroscopyReversed-phase chromatographyFourier transform spectroscopyAnalytical Chemistrydescription
Abstract An on-line gradient reversed phase liquid chromatography–Fourier transform infrared spectrometry (LC–FTIR) method was developed for the determination of fructose, glucose, sucrose and maltose in beverages. Improved chromatographic resolution was obtained using a linear gradient from 75 to 55% (v/v) acetonitrile in water in 15 min. Changes in the background spectra were corrected employing “univariate background correction based on the use of a reference spectra matrix” (UBC-RSM) and using the ratio of absorbance (AR) at 2256 and 2253 cm −1 for the identification of the eluent spectra within the RSM. The method provided limits of detection in the order of 0.75 mg ml −1 . The precision (as relative standard deviation) ranged between 3.3 and 4.1% for glucose and fructose, respectively at a concentration level of 3.0 mg ml −1 . Quantitative recovery values on spiked samples confirmed the accuracy of the method. A set of samples from the Spanish market were analysed to test the suitability of the procedure.
year | journal | country | edition | language |
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2008-12-15 | Talanta |