6533b7ddfe1ef96bd1273ea7

RESEARCH PRODUCT

Modified apparatus for voltage gradient gel electrophoresis.

Maria Rita AsaroVincenzo IzzoRainer Barbieri

subject

Gel electrophoresisChromatographyChemistryOrganic ChemistryAnalytical chemistryVoltage gradientGeneral MedicineFractionationEquipment DesignBiochemistryAnalytical ChemistryElectrophoresisNucleic acidPulsed-field gel electrophoresisElectrophoresis Polyacrylamide GelElectric potentialVoltage

description

We built a modified version of voltage gradient gel electrophoresis system to correct distortions in nucleic acids electrophoretic migration patterns occurring at the edges of the gel when the original voltage gradient apparatus is used. The new device allows correct fractionation of nucleic acids also when electrophoresis is performed at high voltages.

yearjournalcountryeditionlanguage
1999-09-01Journal of chromatography. A
10.1016/s0021-9673(99)00704-9https://pubmed.ncbi.nlm.nih.gov/10519108