6533b7ddfe1ef96bd1273f84
RESEARCH PRODUCT
An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column
Fernando Mauro LançasSteven Ray WilsonTore VehusMeire Ribeiro Da SilvaOle Kristian BrandtzaegElsa Lundanessubject
Capillary actionAnalytical chemistry02 engineering and technologyMass spectrometry01 natural sciencesBiochemistryChemistry Techniques AnalyticalMass SpectrometryAnalytical Chemistrylaw.inventionCrystallawSample preparationFiberSolid phase extractionFiltrationchemistry.chemical_classificationChromatographyChemistrySolid Phase Extraction010401 analytical chemistryOrganic ChemistryGeneral MedicinePolymer021001 nanoscience & nanotechnologyESPECTROMETRIA DE MASSAS0104 chemical sciencesPolystyrenesPolyvinyls0210 nano-technologyChromatography Liquiddescription
An open tubular (OT) sample preparation/separation platform was developed. A multi-channel polymer layer open tubular (mPLOT) solid phase extraction (SPE) column was prepared by wall-coating the 126 channels (8μm inner diameter (ID) each) of a crystal fiber capillary with an organic polymer, namely poly(styrene-co-octadecene-co-divinylbenzene) (PS-OD-DVB). The mPLOT SPE was coupled on-line with a 10μm×2m poly(styrene-co-divinylbenzene) (PS-DVB) OT liquid chromatography column with nanospray mass spectrometry (OTLC-MS). Compared to using monolithic/particle-packed SPEs, mPLOT-SPE-OTLC allowed both fast loading and sufficient refocusing on the OT analytical column of small model compounds (sulfonamides≈300Da). Using automatic filtration/filter back-flushing (AFFL) plumbing, the mPLOT SPE column gave a constant and low back-pressure ≈35bar at 0.5μL/min. Surprisingly large sample volumes (10μL) were possible to be injected using a 12cm mPLOT.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-01-11 |