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RESEARCH PRODUCT

Analysis of eIF4E gene sconfering resistance to Pea seed-borne mosaic virus (PSbMV) and other potyviruses in pea

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Pea seed-borne mosaic virus (PSbMV) belongs among the most frequent viral pathogens causing severe losses in field pea and other legumes. These losses might be prevented by growth of resistant varieties. Resistance to the common P1 strain of PSbMV isconferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus), while to lentil L1 strainis localized on LGII atsbm-2locus. Gene-specific single nucleotide polymorphism and co-dominant amplicon length polymorphism markers were developed. From the comparison of genotype verses phenotype for selection of resistance/susceptibility in F2 plants, there was a 26% discrepancy between the PCR and ELISA-based assays with potentially susceptible heterozygote plants missed. We used qRT-PCR to analyze expression of both resistant and sensitive eIF4E alleles in heterozygotes, sensitive and resistant homozygotes after PSbMV infection, together with evaluation of viral concentration. The novel eIF4E alleles were detected in wider pea germplasm and TILLING mutants and these were virologically tested. We have analyzed eIF4E allelic variation in over 2000 accesions within geographical context. Furthemore, analysis of second sbm-2 locus with eIF4E iso and eIF4G genes as candidate targets, will be presented. It can be expected that information gain in this study might be extended to other legume species, particularly to economically important species of tribus Fabeae or even to subfamily Papilionoideae such as chickpea, lentil, faba bean or soybean where Potyviruses cause problems. Acknowledgements: The work was supported by NAZV QI91A229 project.