6533b7ddfe1ef96bd1274849
RESEARCH PRODUCT
Effect of high hydrostatic pressure on extraction of B-phycoerythrin from Porphyridium cruentum: Use of confocal microscopy and image processing
Ludovic JournauxNathalie CayotRémi PradellesEmmanuel DenimalJean-marie Perrier-cornetPascale WincklerThierry TranCamille LoupiacCéline LafargeJohn Aldo Leesubject
020209 energyHydrostatic pressurePorphyridium cruentumExtraction02 engineering and technologylaw.invention0404 agricultural biotechnologyHigh hydrostatic pressureImage processingConfocal microscopylawSpectrophotometry0202 electrical engineering electronic engineering information engineeringmedicineDenaturation (biochemistry)Confocal laser scanning microscopyB-phycoerythrinmedicine.diagnostic_testbiologyChemistryExtraction (chemistry)04 agricultural and veterinary sciencesbiology.organism_classification040401 food scienceFluorescencePorphyridium cruentumbiology.proteinBiophysicsAgronomy and Crop SciencePhycoerythrin[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processingdescription
International audience; The aim of the study was to extract B-phycoerythrin from Porphyridium cruentum while preserving its structure. The high hydrostatic pressure treatments were chosen as extraction technology. Different methods have been used to observe the effects of the treatment: spectrophotometry and confocal laser scanning microscopy followed by image processing analysis. Image processing led to the generation of masks used for the identification of three clusters: intra, extra and intercellular. All methods showed that high hydrostatic pressure treatments between 50 and 500 MPa failed to extract B-phycoerythrin from Porphyridium cruentum cells. The fluorescence emission was negatively impacted by high hydrostatic pressure treatment from 400 MPa for the extracellular and intercellular cluster and from 500 MPa for the intercellular cluster. These results suggest that high pressure treatments could induce the denaturation of B-phycoerythrin in all clusters but with different intensities depending on the cluster.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-03-01 |