6533b7ddfe1ef96bd1274a19
RESEARCH PRODUCT
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy.
José ÁNgel Picazo-buenoKrzysztof PatorskiMaciej TrusiakJavier GarciaVicente Micósubject
PhysicsMicroscopebusiness.industryNoise reductionBright-field microscopyComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONHolography02 engineering and technologyInterferometric microscopyFrame rate01 natural sciencesMultiplexingAtomic and Molecular Physics and Opticslaw.invention010309 optics020210 optoelectronics & photonicsOpticslaw0103 physical sciences0202 electrical engineering electronic engineering information engineeringbusinessCoherence (physics)description
Hilbert-Huang single-shot spatially multiplexed interferometric microscopy (H2S2MIM) is presented as the implementation of a robust, fast, and accurate single-shot phase estimation algorithm with an extremely simple, low-cost, and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. Altogether, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available nonholographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20×/0.46 NA objective in a regular Olympus BX-60 upright microscope for static, as well as dynamic, samples showing perfect agreement with the results retrieved from a temporal phase-shifting algorithm.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-03-01 | Optics letters |