6533b7ddfe1ef96bd1274a23
RESEARCH PRODUCT
Blend scaffolds with polyaspartamide/polyester structure fabricated via TIPS and their RGDC functionalization to promote osteoblast adhesion and proliferation
Ilenia VitranoFlavia BongiovìVincenzo La CarrubbaGaetano GiammonaGaetano GiammonaFrancesco Carfì PaviaValerio BrucatoGiovanna PitarresiFabio Salvatore Palumbosubject
Materials sciencePolyesters0206 medical engineeringBiomedical EngineeringBiocompatible Materialscyclic RGDC02 engineering and technologyPeptides CyclicPLLACell LineBiomaterialsMiceHydrolysischemistry.chemical_compoundCell AdhesionCopolymerAnimalsCell adhesionMaleimideporous scaffoldCell ProliferationOsteoblastsTissue ScaffoldsMetals and AlloysBiomaterialPHEA021001 nanoscience & nanotechnology020601 biomedical engineeringPolyesterChemical engineeringchemistryCeramics and CompositesMichael reactionSurface modificationTIPSPeptides0210 nano-technologydescription
Target of this work was to prepare a RGDC functionalized hybrid biomaterial via TIPS technique to achieve a more efficient control of osteoblast adhesion and diffusion on the three-dimensional (3D) scaffolds. Starting from a crystalline poly(l-lactic acid) (PLLA) and an amorphous alpha,beta-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-d,l-aspartamide-graft-polylactic acid (PHEA-EDA-g-PLA) copolymer, blend scaffolds were characterized by an appropriate porosity and pore interconnection. The PHEA-EDA-PLA interpenetration with PLLA improved hydrolytic susceptibility of hybrid scaffolds. The presence of free amino groups on scaffolds allowed to tether the cyclic RGD peptide (RGDC) via Michael addition using the maleimide chemistry. Cell culture test carried out on preosteoblastic cells MC3T3-E1 incubated with scaffolds, has evidenced cell adhesion and proliferation. Furthermore, the presence of distributed bone matrix on all scaffolds was evaluated after 70 days compared to PLLA only samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-09-03 |