6533b7ddfe1ef96bd1274a82

RESEARCH PRODUCT

Sequence of a new tRNALeu(U∗AA) from brewer's yeast

Guy DirheimerC. El AdlouniJean DesgresGérard Keith

subject

chemistry.chemical_classificationGeneticsRNA Transfer LeuBase SequencebiologyMolecular Sequence DataSaccharomyces cerevisiaeNucleic acid sequenceProtein primary structureSaccharomyces cerevisiaeGeneral Medicinebiology.organism_classificationBiochemistryYeastHomology (biology)BiochemistrychemistryTransfer RNANucleic Acid ConformationElectrophoresis Gel Two-DimensionalNucleotideLeucineCodon

description

The nucleotide sequence of a new tRNA(Leu)(anticodon U*AA) from Saccharomyces cerevisiae which could recognize exclusively the UUA codon has been determined. Its primary structure is: pGGAGGGUUGm2GCac4CGAGDGmGDCDAAGGCm2(2)GGCAGACmUU*AAm1GA++ + psi CUGUUGGACGGUUGUCCGm5CGCGAGT psi CGm1A(orA)ACCUCGCAUCCUUCACCA. This tRNA has a large extraloop and contains 15 modified nucleotides. So far it is the third isoacceptor tRNA for leucine in yeast. It has 61% homology with tRNA(Leu)(anticodon m5CAA) and 63% homology with tRNA(Leu)(anticodon UAG), the two other known yeast tRNAs(Leu).

yearjournalcountryeditionlanguage
1991-11-01Biochimie
https://doi.org/10.1016/0300-9084(91)90165-w