6533b7ddfe1ef96bd1274ab9
RESEARCH PRODUCT
Transcriptional regulation of nucleoredoxin-like genes takes place on a daily basis in the retina and pineal gland of rats.
Stefanie KunstTanja WolloscheckDebra K. KelleherRainer Spessertsubject
Malemedicine.medical_specialtyPhysiologyCircadian clockLaser Capture MicrodissectionBiologyPineal GlandRetinaPinealocyteRats Sprague-DawleyPineal glandInternal medicineRetinitis pigmentosamedicineAnimalsPhotoreceptor CellsCircadian rhythmRNA MessengerRegulation of gene expressionHomeodomain ProteinsRetinaGenes HomeoboxNuclear ProteinsDarknessmedicine.diseaseSensory SystemsCircadian RhythmRatsEndocrinologymedicine.anatomical_structureGene Expression RegulationDarknessFemalesense organsOxidoreductasesdescription
AbstractThe nucleoredoxin-like gene Nxnl1 (Txnl6) and its paralogue Nxnl2 encode the rod-derived cone viability factors (RdCVF and RdCVF2), which increase the resistance to photooxidative damage and have therapeutic potential for the survival of cones in retinitis pigmentosa. In this study, the transcription of Nxnl genes was investigated as a function of the day/night cycle in rats. The transcript levels of Nxnl1 and Nxnl2 were seen to display daily rhythms with steadily increasing values during the light phase and peak expression around dark onset in preparations of whole retina, photoreceptor cells and—but only in regard to Nxnl1—in photoreceptor-related pinealocytes. The cycling of Nxnl1 but not that of Nxnl2 persisted in constant darkness in the retina. This suggests that daily regulation of Nxnl1 is driven by a circadian clock, whereas that of Nxnl2 is promoted by environmental light. The present data indicate clock- and light-dependent regulations of nucleoredoxin-like genes that may be part of a protective shield against photooxidative damage.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-08-05 | Visual neuroscience |