6533b7ddfe1ef96bd1274b29
RESEARCH PRODUCT
The Drosophila ACP65A cuticle gene: deletion scanning analysis of cis-regulatory sequences and regulation by DHR38.
Jean-philippe CharlesBrigitte QuennedeyMatthieu LestradetA. GirardJosiane AlabouvetteE. GervasioL. HongN. Bruey-sedanosubject
MaleReceptors SteroidTranscription GeneticTransgenelac operonReceptors Cytoplasmic and NuclearBiologyFusion geneP elementAnimals Genetically ModifiedEndocrinologyGeneticsNuclear Receptor Subfamily 4 Group A Member 1AnimalsDrosophila ProteinsEnhancerGeneCrosses GeneticSequence DeletionGeneticsBase SequenceActivator (genetics)fungiPupaCell BiologyDNA-Binding ProteinsGene Expression RegulationRegulatory sequenceInsect ProteinsDrosophilaFemaleTranscription Factorsdescription
The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between −594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene. genesis 43:17–27, 2005. © 2005 Wiley-Liss, Inc.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-08-18 | Genesis (New York, N.Y. : 2000) |