6533b7ddfe1ef96bd12751e9

RESEARCH PRODUCT

Recombinant GM-CSF Induces in Vitro Differentiation of Dendritic Cells from Mouse Bone Marrow

Maria MehligChristoph ScheicherFriedrich SeilerPetra Hintz-obertreisReinhard ZecherKonrad Reske

subject

education.field_of_studyPopulationDendritic cellBiologyIn vitrolaw.inventionCell biologyImmune systemmedicine.anatomical_structurelawRecombinant DNAmedicineBone marrow cultureBone marrowAntigen-presenting celleducation

description

The unprecedented functional capacity of dendritic cells (DC) in sensitizing resting T cells and their role in triggering T dependent immune responses attract increasing interest in this unique accessory cell population. Like macrophages (Mph) DC have been described to originate in the bone marrow (BM) (1). While the cytokine-promoted in vitro differentiation of Mph from BM-cells is well established, a convincing in vitro culture system for propagating mouse DC from BM-cells has not yet been reported. This work demonstrates the differentiation of DC from mouse bone marrow cells by a short term in vitro culture system supplemented with rGM-CSF.

yearjournalcountryeditionlanguage
1993-01-01
https://doi.org/10.1007/978-1-4615-2930-9_45