6533b7ddfe1ef96bd127526b

RESEARCH PRODUCT

Connexin36 (Cx36) expression and protein detection in the mouse carotid body and myenteric plexus

Giuseppa MudòMonica FrinchiFrancesca D’antoniSada Lakshmi TurimellaNatale BelluardoValentina Di LibertoMartin Theis

subject

MalePathologymedicine.medical_specialtyHistologyMousegenetic structuresMyenteric plexusBlotting WesternIleumConnexinBiologySettore BIO/09 - FisiologiaConnexinsMice03 medical and health sciences0302 clinical medicinemedicineAnimalsGap junctionsMyenteric plexus030304 developmental biologyMice Knockout0303 health sciencesReverse Transcriptase Polymerase Chain ReactionGap junctions Carotid body Myenteric plexus Connexin Cx36 MouseCell BiologyGeneral MedicineImmunohistochemistryMolecular biologyMice Inbred C57BLBlotCarotid bodymedicine.anatomical_structureReal-time polymerase chain reactionCx36Knockout mouseImmunohistochemistryCarotid bodysense organs030217 neurology & neurosurgeryImmunostaining

description

AbstractAlthough connexin36 (Cx36) has been studied in several tissues, it is notable that no data are available on Cx36 expression in the carotid body and the intestine. The present study was undertaken to evaluate using immunohistochemistry, PCR and Western blotting procedures, whether Cx36 was expressed in the mouse carotid body and in the intestine at ileum and colon level. In the carotid body, Cx36 was detected as diffuse punctate immunostaining and as protein by Western blotting and mRNA by RT-PCR. Cx36 punctate immunostaining was also evident in the intestine with localization restricted to the myenteric plexus of both the ileum and the colon, and this detection was also confirmed by Western blotting and RT-PCR. All the data obtained were validated using Cx36 knockout mice. Taken together the present data on localization of Cx36 gap-junctions in two tissues of neural crest-derived neuroendocrine organs may provide an anatomical basis for future functional investigations.

yearjournalcountryeditionlanguage
2013-04-01
10.1016/j.acthis.2012.07.005http://hdl.handle.net/10447/75160