6533b7ddfe1ef96bd1275303
RESEARCH PRODUCT
O-Ribosyl-phosphate purine as a constant modified nucleotide located at position 64 in cytoplasmic initiator tRNAsMetof yeasts
Jacques HeitzlerGérar KeithAnne-lise GlasserCharles W. GehrkeJean Desgressubject
chemistry.chemical_classificationRNA Transfer MetbiologyPeriodic AcidSaccharomyces cerevisiaeGuanosine MonophosphateGuanosineRNA Fungalbiology.organism_classificationSaccharomycesMass Spectrometrychemistry.chemical_compoundchemistryBiochemistrySchizosaccharomycesGuanosine monophosphateTransfer RNASchizosaccharomyces pombeGeneticsSpectrophotometry UltravioletNucleotideOxidation-ReductionChromatography High Pressure LiquidSchizosaccharomycesCandidadescription
The unknown modified nucleotide G*, isolated from both Schizosaccharomyces pombe and Torulopsis utilis initiator tRNAs(Met), has been identified as an O-ribosyl-(1"----2')-guanosine-5"-phosphate, called Gr(p), by means of HPLC, UV-absorption, mass spectrometry and periodate oxidation procedures. By comparison with the previously published structure of Ar(p) isolated from Saccharomyces cerevisiae initiator tRNA(Met), the (1"----2')-glycosidic bond in Gr(p) has been postulated to have a beta-spatial conformation. The modified nucleotide Gr(p) is located at position 64 in the tRNA(Met) molecules, i.e. at the same position as Ar(p). Since we have also characterized Gr(p) in Candida albicans initiator tRNA(Met), the phosphoribosylation of purine 64 can be considered as a constant nucleotide modification in the cytoplasmic initiator tRNAs(Met) of all yeast species so far sequenced. Precise evidence for the presence of Gr(p) in initiator tRNAs(Met) of several plants is also reported.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-10-11 | Nucleic Acids Research |