6533b7ddfe1ef96bd1275307

RESEARCH PRODUCT

Selected lactic acid bacteria as a hurdle to the microbial spoilage of cheese: Application on a traditional raw ewes' milk cheese

Rosa GuarcelloLuca SettanniMassimo TodaroNicola FrancescaCiro SanninoRaimondo GaglioStefania Carpino

subject

Food spoilageApplied Microbiology and BiotechnologyDA ESTRELA CHEESEEnterococcus faecalisLactic acid bacteria; Traditional cheese; Raw milk; Pecorinochemistry.chemical_compoundStarterFOODLactic acid bacteriaSettore AGR/18 - Nutrizione E Alimentazione AnimaleFood sciencebiologySTRAINSLactococcus lactisPecorinofood and beveragesTraditional cheeseRaw milkRaw milkbiology.organism_classificationLactic acidchemistryLactococcus garvieaeSAFETYbacteriaBacteriaDA ESTRELA CHEESE; STRAINS; SAFETY; FOODFood ScienceSettore AGR/16 - Microbiologia Agraria

description

To evaluate the efficacy of lactic acid bacteria (LAB) to improve the hygienic safety of a traditional raw milk cheese, the raw ewes’ milk protected denomination of origin (PDO) Pecorino Siciliano cheese was used as a model system. Different Pecorino Siciliano curds and cheeses were used as sources of autochthonous LAB subsequently used as starter and non-starter LAB. These were screened for their acidification capacity and autolysis. Starter LAB showing the best performance were genotypically differentiated and identified: two strains of Lactococcus lactis subsp. lactis were selected. From the nonstarter LAB, Enterococcus faecalis, Lactococcus garvieae and Streptococcus macedonicus strains were selected. The five cultures were used in individual or dual inocula to produce experimental cheeses in a dairy factory for which production was characterised by high numbers of undesirable bacteria. At 5- month of ripening, the experimental cheeses produced with LAB were characterised by undetectable levels of enterobacteria and pseudomonads and the typical sensory attributes.

10.1016/j.idairyj.2013.04.010http://hdl.handle.net/11391/1366599