mPEG-PLGA Nanoparticles Labelled with Loaded or Conjugated Rhodamine-B for Potential Nose-to-Brain Delivery

6533b7ddfe1ef96bd127532c

RESEARCH PRODUCT

mPEG-PLGA Nanoparticles Labelled with Loaded or Conjugated Rhodamine-B for Potential Nose-to-Brain Delivery

Teresa Musumeci Irina Naletova Cristina Satriano Gennara Cavallaro Lorena Maria Cucci Angela Bonaccorso Rosalia Pellitteri Emanuela Fabiola Craparo Alessia Romeo

subject

Pharmaceutical Science olfactory ensheathing cells Blood–brain barrier Article fluorescent dye olfactory ensheathing cells PC12 cell line co-polymers nanomedicine imaging chemistry.chemical_compound Pharmacy and materia medica In vivo medicine Rhodamine B PC12 cell line Cytotoxicity fluorescent dye; olfactory ensheathing cells; PC12 cell line; co-polymers; nanomedicine; imaging Chemistry technology industry and agriculture imaging nanomedicine RS1-441 medicine.anatomical_structure Settore CHIM/09 - Farmaceutico Tecnologico Applicativo Biophysics Nanomedicine co-polymers Nasal administration fluorescent dye Drug carrier Ethylene glycol

description

Nowdays, neurodegenerative diseases represent a great challenge from both the therapeutic and diagnostic points of view. Indeed, several physiological barriers of the body, including the blood brain barrier (BBB), nasal, dermal, and intestinal barriers, interpose between the development of new drugs and their effective administration to reach the target organ or target cells at therapeutic concentrations. Currently, the nose-to-brain delivery with nanoformulations specifically designed for intranasal administration is a strategy widely investigated with the goal to reach the brain while bypassing the BBB. To produce nanosystems suitable to study both in vitro and/or in vivo cells trafficking for potential nose-to-brain delivery route, we prepared and characterized two types of fluorescent poly(ethylene glycol)-methyl-ether-block-poly(lactide-co-glycolide) (PLGA–PEG) nanoparticles (PNPs), i.e., Rhodamine B (RhB) dye loaded- and grafted- PNPs, respectively. The latter were produced by blending into the PLGA–PEG matrix a RhB-labeled polyaspartamide/polylactide graft copolymer to ensure a stable fluorescence during the time of analysis. Photon correlation spectroscopy (PCS), UV-visible (UV-vis) spectroscopies, differential scanning calorimetry (DSC), atomic force microscopy (AFM) were used to characterize the RhB-loaded and RhB-grafted PNPs. To assess their potential use for brain targeting, cytotoxicity tests were carried out on olfactory ensheathing cells (OECs) and neuron-like differentiated PC12 cells. Both PNP types showed mean sizes suitable for nose-to-brain delivery (&lt

year	journal	country	edition	language
2021-09-18	Pharmaceutics

10.3390/pharmaceutics13091508 http://dx.doi.org/10.3390/pharmaceutics13091508