6533b7defe1ef96bd1275c84
RESEARCH PRODUCT
Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice.
Pentti HuovinenEveliina MunukkaRaine ToivonenHeikki KainulainenMaija HollménAnniina KeskitaloSirpa JalkanenSatu Pekkalasubject
rasvahapotPathology and Laboratory Medicinerasvat (orgaaniset yhdisteet)ruokavaliotBiochemistryMiceAnimal CellsFibrosislcsh:ScienceImmune ResponseConnective Tissue CellsChemical Reactionsta3141ta3142Lipids3. Good healthPhysical sciencesAdiponectinCellular Typesmedicine.medical_specialtyfatsLipolysisImmunologySubcutaneous FatrasvakudoksetMonomers (Chemistry)glycerolDiet High-Fatta311103 medical and health sciencesSigns and SymptomsEnterobacter cloacaeLipolysisPolymer chemistrylcsh:RBiology and Life SciencesHypertrophymedicine.diseaseReceptor InsulinMice Inbred C57BLBiological Tissue030104 developmental biologyEndocrinologylihavuuslcsh:QGlycerol0301 basic medicinePhysiologyLiver cytologysuolistomikrobistolcsh:MedicineAdipose tissueGut floraMedicine and Health SciencesAdipocytesenterobakteerit2. Zero hungerrasvatMultidisciplinarygastrointestinal microbiotatulehdusbiologyHydrolysisadipose tissueChemistryPhysiological ParametersLiverConnective Tissueembryonic structuresFemaleAnatomymedicine.symptomResearch Articleanimal structuresadipocytesInflammationInsulin resistanceEnterobacteriaceaeDiagnostic MedicineInternal medicinemedicineAnimalsObesityTriglyceridesNutritionurogenital systembusiness.industryBody WeightCell Biologybiology.organism_classificationDietToll-Like Receptor 5Gene Expression RegulationinflammationlipolysisdietbusinessEnterobacter cloacaedescription
Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E. cloacae treatment groups. The E. cloacae treatment group received live bacterial cells in PBS intragastrically twice a week, every other week for 13 weeks. Both control groups received PBS intragastrically. After the 13- week treatment period, the mice were sacrificed for gene and protein expression and functional analyses. Our results show that E. cloacae administration increased subcutaneous fat mass and the relative proportion of hypertrophic adipocytes. Both subcutaneous and visceral fat had signs of decreased insulin signaling and elevated lipolysis that was reflected in higher serum glycerol levels. In addition, E. cloacae -treated mice had significantly higher hepatic AST and AST/ALT ratio, and their liver histology indicated fibrosis, demonstrating that E. cloacae subsp. cloacae administration promotes hepatic damage in HFD fed mice. peerReviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-05-01 | PLoS ONE |