6533b7defe1ef96bd1275c89

RESEARCH PRODUCT

Changes in plasma lecithin: cholesterol acyltransferase activity, HDL2, HDL3 amounts and compositions in patients with chronic renal failure after different times of hemodialysis

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The aim of this study was to investigate the effect of hemodialysis duration on HDL(2) and HDL(3) compositions and lecithin:cholesterol acyltransferase (LCAT) activity in 58 patients on acetate hemodialysis using cuprophane membrane, after different periods of dialysis. Patients were divided into three groups-GI1 year, GII 1-5 years, GIII 5-13 years of dialysis and were compared with 22 controls. Increase by 34% of triacylglycerols (TG) was noted in GI and by 36% in GII versus GI. Hypertriglyceridemia was correlated with hemodialysis duration (HD) (r=0.75, P0.05). The LCAT activity decrease by 25% was noted in GII versus GI (P0.05) and by 45% in GIII versus GI (P0.01), this activity was negligible in 33% of GII and 39% of GIII. LCAT activity was negatively correlated with HD (r=-0.80, P0.001). Hemodialysis duration did not influence HDL(3) and HDL(2) amounts, HDL(3)-phospholipids (PL), HDL(3) and HDL(2)-cholesteryl esters (CE) and HDL(2)-apolipoproteins. However, an increase by 56% in HDL(2)-PL was noted in GIII versus GI and GII (P0.01). HDL(2)-unesterified cholesterol (UC) were 2-fold higher in GIII than GII and GI (P0.01). HDL(2)-TG were 2.2-fold higher in GII and 2.4-fold in GIII than GI (P0.001). HDL(3)-apolipoproteins were 1.5-fold and 1.8-fold lower in GI than GII (P0.05) and GIII (P0.01), respectively. An increase in HDL(3)-UC by 20% in GII and 33% in GIII versus GI (P0.01) was noted. HDL(3)-TG increase by 66% was noted in GII and GIII versus GI (P0.05). We conclude, that the long-term hemodialysis does not act on lipid anomalies following CRF. Alterations in HDL composition contribute to the reduced efficacy of reverse cholesterol transport, which is a risk factor for cardiovascular disease.