6533b7defe1ef96bd1275ca8
RESEARCH PRODUCT
Constitutive hsp70 is essential to mitosis during early cleavage of Paracentrotus lividus embryos: The blockage of constitutive hsp70 impairs mitosis
Giovanni SpinelliFranco PallaCecilia AgueliFabiana GeraciD. CascinoGiovanni GiudiceGabriella Sconzosubject
Time FactorsGrowth InhibitorMicroinjectionsCell divisionTime FactorSea UrchinCleavage Stage OvumBiophysicsMitosisCleavage (embryo)BiochemistryParacentrotus lividuschemistry.chemical_compoundbiology.animalAnimalsHSP70 Heat-Shock ProteinsSea urchin embryoMitosisMicroinjectionSea urchinMolecular BiologyConstitutive hsp70HSP70 Heat-Shock ProteinbiologyDose-Response Relationship DrugAnimalNocodazoleCell Biologybiology.organism_classificationMitosiGrowth InhibitorsMicroinjectionCell biologyNocodazolechemistryBiophysicCentrosomeSea UrchinsFertilizationembryonic structuresdescription
Localization of constitutive hsp70 in eggs and early embryos of sea urchin Paracentrotus lividus is shown by means of in situ immunostaining. An accumulation of this protein is shown in the mitotic structures (asters, spindles and centrosomes). Microinjection of anti-hsp70 antibodies into eggs causes impairment of formation of mitotic structures and of cell division. This impairment goes from a complete mitotic block, to irregular mitotic apparatus formation with irregular cleavage, depending upon the antibody concentration. The localization of hsp70 after antibody microinjection is also described. Blockage of mitotic apparatus formation by nocodazole also blocks the concentration of hsp70 molecules observed in nontreated eggs. That the constitutive hsp70 plays a role in sea urchin mitosis is indicated.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-06-01 |