6533b7defe1ef96bd1275db7

RESEARCH PRODUCT

Performance evaluation of a newly developed molecular assay for the accurate diagnosis of gastroenteritis associated with norovirus of genogroup II

Noemi UroneVincenzo CappaFloriana BonuraSimona De GraziaSara Li MuliGiovanni M. GiammancoA. Pepe

subject

0301 basic medicineMalemedicine.medical_specialtySettore MED/07 - Microbiologia E Microbiologia ClinicaAdolescentGenotype030106 microbiologyDiagnostic accuracyBiologymedicine.disease_causeReal-Time Polymerase Chain ReactionSensitivity and SpecificityFeces03 medical and health sciencesMedical microbiologyVirologyGenotypemedicineHumansChildCaliciviridae InfectionsNoroviruCaliciviridae InfectionGastroenteritiReverse Transcriptase Polymerase Chain ReactionNorovirusInfantGeneral MedicineAcute gastroenteritisbacterial infections and mycosesVirologyGastroenteritis030104 developmental biologyCaliciviridae InfectionsFully automatedChild PreschoolNorovirusFeceFemaleHuman

description

The performance of a newly proposed fully automated cassette-based sample-to-results solution for norovirus (NoV) detection, InGenius Norovirus ELITe MGB®, was evaluated. A total of 120 selected archival stool samples from children hospitalized for acute gastroenteritis were used to compare the results to a reference real-time RT-PCR. The InGenius NoV assay showed optimal diagnostic accuracy (sensitivity, 100%; specificity, 95.7%) and was able to correctly detect the entire wide panel of epidemiologically relevant genotypes tested. These preliminary results suggest that the InGenius NoV assay can be recommended as a valuable method for accurate diagnosis of NoV GII infection in epidemic and sporadic gastroenteritis.

yearjournalcountryeditionlanguage
2018-06-15
10.1007/s00705-018-4010-8http://hdl.handle.net/10447/332940