6533b7defe1ef96bd12767c1
RESEARCH PRODUCT
Amino acids requirements of measles virus in HeLa cells.
N. RomanoG. Scarlatasubject
OrnithinePlating efficiencyTime FactorsArginineViral Plaque AssayBiologyArginineVirus ReplicationVirusMeasles viruschemistry.chemical_compoundMethionineVirologyHumansAmino Acidschemistry.chemical_classificationMethionineStereoisomerismGeneral Medicinebiology.organism_classificationVirologyAmino acidCulture MediaGlutaminechemistryMeasles virusCitrullineIsoleucineHeLa Cellsdescription
A low plating efficiency for measles virus was obtained when arginine, threonine and isoleucine were omitted from Eagle's basal medium and a stimulatory effect on the plaque production was produced by increasing the concentration up to 0.5 mm of arginine and glutamine. However, of all amino acids in Eagle's basal medium, only methionine was found to be essential for the synthesis of infectious progeny virus. Omission of any one of the other amino acids from the culture medium permitted limited virus production, but none whatever could be detected in the absence of mediummethionine. Moreover, when methionine was restored to infected cultures after 24 hours of deprivation, release of virus became soon detectable and the titre increased rapidly.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1973-12-01 | Archiv fur die gesamte Virusforschung |