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RESEARCH PRODUCT

Triiodothyronine-Induced Shortening of Chromatin Repeat Length in Neurons Cultured in a Chemically Denned Medium

Italia Di LiegroA. CestelliRoberto GristinaGiuseppe SalemiDonatella FerraroGiovanni SavettieriDaniele Castiglia

subject

Neuronal terminal differentiationTime FactorsCellular differentiationBiologySettore BIO/19 - Microbiologia GeneraleChromatin structureBiochemistryCellular and Molecular NeuroscienceSettore BIO/10 - BiochimicamedicineAnimalsNucleosomeMitosisCells CulturedCerebral CortexNeuronsGeneticsNucleosomal Repeat LengthTriiodothyronineDNAChromatinCulture MediaRatsChromatinCell biologyChemically denned medium)Chemically defined mediummedicine.anatomical_structurenervous systemTriiodothyronineSettore MED/26 - NeurologiaNeuron

description

Abstract: At the time of terminal differentiation, mammalian cortical neurons undergo a dramatic change in the structural organization of their chromatin: the nucleosomal repeat length shortens from ∼200 base pairs in fetuses to a value of 165 base pairs after birth. These events occur several days after the end of neuronal proliferation. Previously, we reported that rat cortical neurons cultured in a very selective synthetic medium were not yet programmed to these events at the end of mitotic cycles. Herein, we report that addition of triiodothyronine to neuronal cultures induces a shortening of the chromatin repeat length comparable to the natural one. Copyright © 1987, Wiley Blackwell. All rights reserved

yearjournalcountryeditionlanguage
1987-04-01Journal of Neurochemistry
https://doi.org/10.1111/j.1471-4159.1987.tb05626.x