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RESEARCH PRODUCT
Prevention of the acute neurotoxic effects of phenytoin on rat peripheral nerve by H7, an inhibitor of protein kinase C.
Francisco J. PuertasSantiago GrisoliaCarlos HermenegildoMaría Dolores MiñanaVicente FelinoAngel RayaJuan GallegoJoaquín RomáFrancisco J. Romerosubject
PhenytoinMalemedicine.medical_treatmentIntraperitoneal injectionPharmacologyToxicologyNeuromuscular junctionPiperazines1-(5-Isoquinolinesulfonyl)-2-MethylpiperazinemedicineAnimalsPeripheral NervesNa+/K+-ATPaseRats WistarProtein kinase CProtein Kinase CbiologyChemistryIsoquinolinesSciatic NerveElectric StimulationRatsElectrophysiologymedicine.anatomical_structureAnticonvulsantEnzyme inhibitorAnesthesiaPhenytoinbiology.proteinSciatic nerveSodium-Potassium-Exchanging ATPaseInjections Intraperitonealmedicine.drugdescription
Abstract The neurotoxic effects of a single dose of phenytoin (150 mg/kg body weight) alone or 30 min after H7 (a protein kinase C inhibitor) injection (20 mg/kg body weight) were investigated in terms of peripheral neuromuscular function and Na + ,K + -ATPase activity of the sciatic nerve. This intraperitoneal injection of phenytoin induced complete blockade of muscle action potentials in the dorsal segmental muscles of the rat tail evoked by electric stimulation of the caudal nerve and a 40% decrease in the Na + ,K + -ATPase activity of the rat sciatic nerve when compared with control values, measured as the difference between total and ouabain-insensitive ATPase activity. Prior administration of H7 resulted in the complete prevention of both effects. Implication of protein kinase C inhibition in phenytoin neurotoxicity are discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-11-01 | Toxicology |