Saldūdens dūņu mikroorganismu populāciju dinamika jēlglicerīna fermentācijas laikā

6533b7defe1ef96bd1276b84

RESEARCH PRODUCT

Saldūdens dūņu mikroorganismu populāciju dinamika jēlglicerīna fermentācijas laikā

Matīss Paiders

subject

saldūdens dūņas fermentācija qPCR Firmicutes Bioloģija jēlglicerīns

description

Anaerobās fermentācijas ceļā var lietderīgi izmantot jēlglicerīnu, biodīzeļa ražošanas blakusproduktu, iegūstot produktus ar pievienotu vērtību, kas ļauj padarīt biodīzeļa ražošanu ekonomiski izdevīgāku. Šajā darbā tika pētīta mikroorganismu populāciju dinamika jēlglicerīna anaerobās fermentācijas laikā, izmantojot saldūdens dūņas kā inokulātu. Fermentāciju veica 20°C un 37°C temperatūrā 4 un 8 dienas. Noteica izdalītās gāzes tilpumu, kā arī barotņu organisko vielu daudzumu un sastāvu, nosakot ķīmiskā skābekļa patēriņu un veicot šķidruma hromatogrāfiju. Pētīja mikroorganismu populāciju dinamiku, veicot qPCR ar dažādiem baktēriju taksoniem specifiskiem 16S rDNS praimeru pāriem, kā arī veicot kultivējamo mikroorganismu daudzuma noteikšanu un identifikāciju. Pēc 4 dienu fermentācijas tika konstatēta pilnīga barotnēs esošā glicerīna utilizācija, saražojot gāzes, organiskās skābes un spirtus. Lielākais gāzes tilpums tika iegūts pie 37°C temperatūras. Tika aprēķināts etanola iznākums 0,23 g/g glicerīna. Jēlglicerīna fermentācijā tika novērots izteikts Firmicutes tipa baktēriju pieaugums, dominējot Clostridia un Bacilli klasēm.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/48266

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