6533b81ffe1ef96bd127730f
RESEARCH PRODUCT
Morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men
Pilar SantolariaTeresa CarreteroJosé M BernéAraceli BonoPilar RecreoCarles SolerJesús Yánizsubject
0301 basic medicineAdultMalesperm morphometry by computer-assisted sperm morphometry analysisendocrine systemsplit ejaculateUrologyKinematicsInvited Original ArticleBiologylcsh:RC870-923Andrology03 medical and health sciencesYoung Adult0302 clinical medicinemanHumansCell ShapeSperm motilityreproductive and urinary physiology030219 obstetrics & reproductive medicineurogenital systemGeneral Medicineman; sperm morphometry by computer-assisted sperm morphometry analysis; sperm motility by computer-assisted sperm analysis; sperm subpopulations; split ejaculatelcsh:Diseases of the genitourinary system. UrologySpermSpermatozoaMotile spermatozoaSemen Analysis030104 developmental biologysperm motility by computer-assisted sperm analysisSperm Motilitysperm subpopulationsdescription
This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH [35.6% of all motile spermatozoa], with circular trajectories [32.0%], and rapid with high ALH [32.4%]), and three morphometric subpopulations (large-round [33.9% of all spermatozoa], elongated [32.0%], and small [34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-09-01 | Asian Journal of Andrology |