6533b81ffe1ef96bd1277bf2
RESEARCH PRODUCT
Optical sectioning by two-pinhole confocal fluorescence microscopy.
Manuel Martínez-corralVassilios SarafisMaría Teresa Caballero CaballeroC. Ibáñez-lópezsubject
Fluorescence-lifetime imaging microscopyMaterials scienceMicroscopy ConfocalOptical sectioningbusiness.industryConfocalScanning confocal electron microscopyGeneral Physics and AstronomyCell BiologyModels TheoreticalImage Enhancementlaw.inventionOpticsMicroscopy FluorescenceStructural BiologyConfocal microscopylawLight sheet fluorescence microscopySubtraction TechniqueMicroscopyGeneral Materials SciencePinhole (optics)businessdescription
A two-pinhole axially superresolving confocal fluorescence imaging system is presented. Based on the concept of subtractive incoherent imaging, the system described here is equipped with a zero-focus complex-transmittance pupil filter in one of the collector paths. The optical sectioning capacity of the system is 25% superior to that of a free-pupil one-pinhole instrument.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-08-23 | Micron (Oxford, England : 1993) |