6533b81ffe1ef96bd1277e04
RESEARCH PRODUCT
Ethanol cycle in an ethanologenic bacterium
Nina GalininaU MarjutinaM. TomaUldis Kalneniekssubject
NADH channelingBiophysicsRespiratory chainBiochemistryZymomonas mobilischemistry.chemical_compoundStructural BiologyGeneticsGlycolysisEthanol metabolismMolecular BiologyAlcohol dehydrogenaseZymomonasEthanolEthanolbiologyFutile cycleRespirationZymomonas mobilisAlcohol dehydrogenaseAcetaldehydeCell BiologyNADbiology.organism_classificationAerobiosisIsoenzymeschemistryBiochemistryFutile cycleChemostatbiology.proteinOxidation-Reductionhormones hormone substitutes and hormone antagonistsdescription
AbstractA novel redox cycle is suggested, performing interconversion between acetaldehyde and ethanol in aerobically growing ethanologenic bacterium Zymomonas mobilis. It is formed by the two alcohol dehydrogenase (ADH) isoenzymes simultaneously catalyzing opposite reactions. ADH I is catalyzing acetaldehyde reduction. The local reactant ratio at its active site probably is shifted towards ethanol synthesis due to direct channeling of NADH from glycolysis. ADH II is oxidizing ethanol. The net result of the cycle operation is NADH shuttling from glycolysis to the membrane respiratory chain, and ensuring flexible distribution of reducing equivalents between the ADH reaction and respiration.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-07-04 | FEBS Letters |