6533b81ffe1ef96bd127853d

RESEARCH PRODUCT

Anomale röntgenstreuung zur erforschung makromolekularer strukturen

subject

description

The possibilities of anomalous X-ray scattering for the investigation of semi- or noncrystalline macromolecular structures are discussed on the grounds of the first successful measurements of anomalous scattering of iron in dissolved hemoglobin. As anomalous scattering of this kind of material is limited to the near vicinity of the X-ray absorption edges, the use of synchrotron radiation appears to be obligatory. The anomalous dispersion of the atomic form factors slightly increases with the atomic number. Anomalous dispersion is nearly doubled when going from the K-absorption edge to the LIII-absorption edge and from there to the Mv- absorption edge. Therefore, the use of anomalous dispersion in the soft X-ray region is quite promissing. With the planned extension of the spectral range accessible to X-ray diffractometers from 3 A to 6 A, one would get hold of the anomalous scattering of very important elements in macromolecular structures, like phosphorus, sulfur, and chlorine.