6533b81ffe1ef96bd1278750

RESEARCH PRODUCT

Red fruit (Pandanus conoideus Lam) oil stimulates nitric oxide production and reduces oxidative stress in endothelial cells

Huige LiSolveig HasselwanderChristian SchirraNing XiaUlrich Förstermann

subject

0301 basic medicineEndothelial cellsMedicine (miscellaneous)VasodilationMouse aorta030204 cardiovascular system & hematologyPharmacologymedicine.disease_causeNitric oxide03 medical and health scienceschemistry.chemical_compoundPandanus conoideus Lam0302 clinical medicineEnosmedicineTX341-641Nutrition and DieteticsbiologyNutrition. Foods and food supplyNitric oxidePandanus conoideusbiology.organism_classificationComet assay030104 developmental biologychemistryOxidative stressPhosphorylationEndothelial nitric oxide synthaseReactive oxygen speciesOxidative stressFood Science

description

Abstract Red fruit oil (RFO) is used in traditional medicine for the treatment of a number of diseases. However, evidence for the biological effects and action mechanisms is still lacking. In the present study, we show for the first time that RFO stimulated the phosphorylation of the endothelial nitric oxide synthase (eNOS) and enhanced the NO production in human endothelial cells. In isolated mouse aorta, RFO induced a vasodilation, with a significant effect evident at a concentration as low as 1:100,000 dilution. The RFO-induced vasodilation could be completely prevented by eNOS inhibition, indicating that RFO contains highly potent substances stimulating eNOS activity. In addition, RFO reduced oxidative stress in endothelial cells, as demonstrated with three different methods. In the comet assay, RFO significantly decreased oxidative DNA damage induced by genotoxicants. These results suggest that enhanced endothelial NO production and reduced oxidative stress may represent part of the molecular mechanisms responsible for the therapeutic effects of RFO.

yearjournalcountryeditionlanguage
2018-12-01Journal of Functional Foods
https://doi.org/10.1016/j.jff.2018.10.014