6533b81ffe1ef96bd127886e
RESEARCH PRODUCT
Epidemiology ofSalmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources
Villafrate MrA. NastasiCaterina Mamminasubject
DNA BacterialSalmonella typhimuriumSerotypeSalmonellaSwineEpidemiologymedicine.disease_causeDNA RibosomalMicrobiologyBirdsRibotypingDogsmedicineAnimalsHumansTypingRibosomal DNAGeneticsbiologyAnimal SourcesStrain (biology)Ruminantsbiology.organism_classificationDNA FingerprintingEnterobacteriaceaeBacterial Typing TechniquesInfectious DiseasesHedgehogsCatsRabbitsResearch Articledescription
SUMMARYSalmonella typhimuriumis the most frequently identified serovar ofSalmonellain Italy. This serovar is characterized by the widespread dissemination among human and non-human sources of phenotypically and genetically well-differentiated clones.In this study 457 strains ofS. typhimuriumisolated in Italy in the years 1982–91 from human and animal sources were submitted to characterization by the rDNA fingerprinting technique. Application of this typing method, after digestion of chromosomal DNA withHincII endonuclease, confirmed the greatest genetic differentiation of clones ofS. typhimurium, allowing reliable identification of 45 rDNA patterns linked into 9 major clusters. rDNA pattern clusters or ribotypes specific to man were not recognized, whereas some rDNA patterns were characteristically related to ducks, pigeons and pet birds. The ribotyping results for isolates from animal hosts suggest that pig and cattle are the main source of human infection.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-06-01 | Epidemiology and Infection |