6533b81ffe1ef96bd1278f5d

RESEARCH PRODUCT

The Effect of Temperature and Radical Protection on the Photoinhibition of Spinach Thylakoids

Birgit BöthinAloysius WildMichael Richter

subject

PhotoinhibitionbiologyChemistryVitamin Emedicine.medical_treatmentGlutathionebiology.organism_classificationElectron transport chainchemistry.chemical_compoundCatalaseThylakoidbiology.proteinmedicineBiophysicsSpinachAtrazine

description

Photoinhibition of spinach thylakoids was studied by examination the inactivation of different parts of the electron transport chain, the decline of the variable fluorescence and the loss of atrazine binding sites. The results obtained after photoinhibition at 20° C and 0° C revealed an inactivation at the QB-site of the D1-protein as the first event in the course of photoinhibition. The natural antioxidants glutathione and ascorbate as well as the enzymes SOD and catalase diminished photoinhibition to similar extents. Further protection was achieved through combination of both radical defense systems. In addition to the radical scavenging properties glutathione and ascorbate have reducing abilities towards oxidized vitamin E preventing thus damage within the thylakoid membrane.

yearjournalcountryeditionlanguage
1992-01-01
https://doi.org/10.1007/978-1-4615-3366-5_64