6533b820fe1ef96bd1279baf
RESEARCH PRODUCT
Evidence for essential primary amino groups in a bacterial coupling factor F1ATPase.
Klaus DoseHelmut PetersSergio Risisubject
chemistry.chemical_classificationAdenosine TriphosphatasesPrimary (chemistry)Binding SitesChemistryStereochemistryEffectorCell MembraneBiophysicsCell BiologyBiochemistryMicrococcusCoupling (electronics)Structure-Activity RelationshipEnzymeBiochemistrySolubilityPyridoxal PhosphateAtpase activityReactivity (chemistry)Amino AcidsMolecular Biologydescription
Abstract We have found that the binding of pyridoxal-5′-phosphate to 6 primary amino groups leads to the inactivation of the enzyme. A preferential reaction of pyridoxal-5′-phosphate with the α-subunits of this enzyme can be demonstrated. The reactivity of the amino groups is influenced by various effectors. In the presence of ATP the inhibition of the ATPase activity is noncompetitive.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1980-12-16 | Biochemical and biophysical research communications |