6533b820fe1ef96bd1279bdd

RESEARCH PRODUCT

Conversion of the Ca2+-ATPase from Rhodospirillum rubrum into a Mg2+-dependent enzyme by 1,N6-etheno ATP

Klaus DoseHans-jochen SchäferHans Werner Müller

subject

ATPaseBiophysicsPhotophosphorylationCalcium-Transporting ATPasesRhodospirillum rubrumBiochemistrychemistry.chemical_compoundAdenosine TriphosphateMagnesiumMolecular BiologyEdetic Acidchemistry.chemical_classificationbiologyATP synthaseChemiosmosisCell MembraneRhodospirillum rubrumCell Biologybiology.organism_classificationKineticsEnzymeBiochemistrychemistrybiology.proteinNucleoside triphosphateOligomycinsATP synthase alpha/beta subunitsEthenoadenosine TriphosphateProtein Binding

description

Nucleoside triphosphate hydrolysis of R.rubrum ATPase complexes can be changed from Ca2+-dependence to Mg2+-dependence by replacing ATP with 1,N6-etheno ATP. Four ATPase complexes which have been prepared by different procedures hydrolyze ATP and 1,N6-etheno ATP at different rates in dependence on the added metal ions. These differences allow an easy distinction of the various enzyme forms.

yearjournalcountryeditionlanguage
1980-08-01Biochemical and Biophysical Research Communications
https://doi.org/10.1016/0006-291x(80)91587-9