IP-10 is an accurate biomarker for the diagnosis of tuberculosis in children.

6533b820fe1ef96bd1279c72

RESEARCH PRODUCT

IP-10 is an accurate biomarker for the diagnosis of tuberculosis in children.

Carlos Rodrigo Vicenç Ausina Vicenç Ausina Cristina Prat Cristina Prat Neus Altet Nuria Diez Morten Ruhwald José Domínguez José Domínguez Amparo Escribano Irene Mialdea Irene Latorre Irene Latorre Mar Serra-vidal Mar Serra-vidal Irma Casas Jessica Diaz Jessica Diaz

subject

Microbiology (medical)Male medicine.medical_specialty Tuberculosis Adolescent T-Lymphocytes Enzyme-Linked Immunosorbent Assay Interferon-gamma Latent Tuberculosis Internal medicine Active tb medicine Humans Tuberculosis Significant risk Child Retrospective Studies Antigens Bacterial Latent tuberculosis business.industry Active tuberculosis medicine.disease Healthy Volunteers Chemokine CXCL10 Infectious Diseases Child Preschool Immunology Biomarker (medicine)Cytokines Female business Biomarkers

description

Summary Objective Performance of IFN-γ assays in children is compromised. Therefore, we investigated the utility of IP-10 for the detection of active tuberculosis (TB) and latent tuberculosis infection (LTBI) diagnosis in children; comparing its positivity with QuantiFERON-TB Gold In-Tube (QFN-G-IT) and T-SPOT.TB. Methods We studied 230 children from three groups: active TB, screening (healthy children without known exposure to active TB patient screened at school or by their paediatrician) and contact-tracing studies. IFN-γ release was determined by QFN-G-IT and T-SPOT.TB. IP-10 was detected in QFN-G-IT supernatants by ELISA. Results When combining QFN-G-IT and IP-10 assays, positive results improved significantly from 38.3% in QFN-G-IT and 33.9% in IP-10 to 41.3%. Age and type of contact were significant risk factors associated with positive QFN-G-IT and IP-10 results. IP-10 levels after antigen-specific stimulation were significantly higher in comparison to IFN-γ levels. Correlation between the three assays was good ( κ = 0.717–0.783). Conclusions IP-10 cytokine is expressed in response to TB specific-antigens used in QFN-G-IT. In conclusion, the use of IFN-γ T-cell based assays in combination with an additional IP-10 assay detection could be useful for diagnosing active TB and LTBI in children.

year	journal	country	edition	language
2014-03-11	The Journal of infection

10.1016/j.jinf.2014.06.013 https://pubmed.ncbi.nlm.nih.gov/24975172