6533b820fe1ef96bd127a553

RESEARCH PRODUCT

Resolution enhancement in integral microscopy by physical interpolation

J.c. BarreiroManuel Martínez-corralEmilio Sánchez-ortigaAnabel LlavadorGenaro Saavedra

subject

Point spread functionIntegral imagingComputer sciencebusiness.industryResolution (electron density)ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONImage processingcomputer.software_genreArticleAtomic and Molecular Physics and OpticsBiological specimenOpticsMicroscopyData miningbusinesscomputerImage resolutionBiotechnologyInterpolation

description

Integral-imaging technology has demonstrated its capability for computing depth images from the microimages recorded after a single shot. This capability has been shown in macroscopic imaging and also in microscopy. Despite the possibility of refocusing different planes from one snap-shot is crucial for the study of some biological processes, the main drawback in integral imaging is the substantial reduction of the spatial resolution. In this contribution we report a technique, which permits to increase the two-dimensional spatial resolution of the computed depth images in integral microscopy by a factor of √2. This is made by a double-shot approach, carried out by means of a rotating glass plate, which shifts the microimages in the sensor plane. We experimentally validate the resolution enhancement as well as we show the benefit of applying the technique to biological specimens.

https://doi.org/10.1364/boe.6.002854