6533b820fe1ef96bd127a596
RESEARCH PRODUCT
B cell immunosenescence: different features of naive and memory B cells in elderly.
Matteo BulatiSilvio BuffaGiuseppina CandoreSalvatore VitelloMariavaleria PellicanòDeborah K. Dunn-waltersCalogero CarusoYu-chang WuGiuseppina Colonna-romanosubject
AdultAgingNaive B cellSomatic hypermutationImmunoglobulinsInflammationBiologyLymphocyte ActivationElderlymedicineHumansCytokineB cellCellular SenescenceAgedSettore MED/04 - Patologia GeneraleAged 80 and overB-LymphocytesHypermutationIonomycinGerminal centerImmunosenescenceMiddle AgedMemory B cellsInterleukin-10B-1 cellInterleukin 10medicine.anatomical_structureImmunologyTetradecanoylphorbol AcetateGeriatrics and GerontologyGerontologyCell agingImmunologic Memorydescription
Elderly people show a reduced protection against new infections and a decreased response to vaccines as a consequence of impairment of both cellular and humoral immunity. In this paper we have studied memory/naive B cells in the elderly, evaluating surface immunoglobulin expression, production of the pro- and anti-inflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-10, and presence of somatic hypermutation, focusing on the IgG(+)IgD(-)CD27(-) double negative (DN) B cells that are expanded in the elderly. Our results show that naive B cells from young donors need a sufficiently strong stimulus to be activated "in vitro", while naive B cells from old subjects are able to produce IL-10 and TNF-α when stimulated "physiologically" (α-CD40/IL-4), suggesting that these cells might play a role in the control of the immuno-inflammatory environment in the elderly. In addition, in the elderly there is an accumulation of DN B cells with a reduced rate of somatic hypermutation. Thus, DN B lymphocytes may be exhausted cells that are expanded and accumulate as a by-product of persistent stimulation or impaired germinal center formation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-08-31 | Biogerontology |