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RESEARCH PRODUCT
Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water
Rosa AznarEsperanza GarayCovadonga R. Ariassubject
DNA BacterialMolecular Sequence DataVibrio vulnificusPolymerase Chain ReactionSensitivity and SpecificityApplied Microbiology and Biotechnologylaw.inventionMicrobiologylawVibrionaceae23S ribosomal RNAAnimalsRibosomal DNAPolymerase chain reactionDNA PrimersVibrioBase SequenceEcologybiologyFishesRibosomal RNAbiology.organism_classificationMolecular biologyVibrioRNA Ribosomal 23SEvaluation Studies as TopicGenes BacterialWater MicrobiologyNested polymerase chain reactionResearch ArticleFood ScienceBiotechnologydescription
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-09-01 |