6533b821fe1ef96bd127ad02

RESEARCH PRODUCT

RAPID LIQUID CHROMATOGRAPHIC DETERMINATION OF TETRACYCLINES IN ANIMAL FEEDS USING A SURFACTANT SOLUTION AS MOBILE PHASE

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ABSTRACT A chromatographic procedure was developed for the determination of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and minocycline (MINO) in animal feeds. Clear analyte-rich extracts were obtained using a 1 : 1 acetonitrile/water mixture buffered at pH 3. The extracts were injected into a conventional unprotected C18 chromatographic column and eluted with a mobile phase of 0.05 M sodium dodecyl sulfate/5% 1-butanol/0.01 M oxalic acid at pH 3. Good resolution was achieved for the five compounds, whereas OTC and TC coeluted with an optimized aqueous-organic mobile phase of methanol/acetonitrile/0.01 M oxalic acid at pH 3. Mean recoveries from spiked feed samples were 79, 83, 86, 87 and 95% for OTC, TC, CTC, DC and MINO, respectively, in the 15–100 µg g−1 concentration range. Limits of detection were in the range 0.1–0.4 µg mL−1. No interference was found from other antibiotics (penicillins G and V, amoxicillin, chloramfenicol, thiamfenicol, erythromycin and trime...