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RESEARCH PRODUCT
Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography
H.-j. SchäferK.k. UngerG. RathgeberWerner MüllerG. JilgeU. Essersubject
Solvent strengthChromatographyResolution (mass spectrometry)Critical parameterChemistryOrganic ChemistryGeneral MedicinePorosityBiochemistryHigh-performance liquid chromatographyAnion exchangerAnalytical ChemistryIondescription
Abstract The linear solvent strength model of Snyder was applied to describe fast protein separations on 2.1-μm non-porous, silica-based strong anion exchangers. It was demonstrated on short columns packed with these anion exchangers that (i) a substantially higher resolution of proteins and nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers; (ii) the low external surface area of the non-porous anion exchanger is not a critical parameter in analytical separations and (iii) μg-amounts of enzymes of high purity and full biological activity were isolated.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-01-01 | Journal of Chromatography A |