Isolation of Zebrafish Gonads for RNA Isolation

6533b821fe1ef96bd127b6a9

RESEARCH PRODUCT

Isolation of Zebrafish Gonads for RNA Isolation

subject

endocrine system Gonad urogenital system Piwi-interacting RNA Biology biology.organism_classification Germline Cell biology medicine.anatomical_structure medicine RNA extraction Animal species Zebrafish Germ cell Function (biology)

description

Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.

year	journal	country	edition	language
2013-10-07

https://doi.org/10.1007/978-1-62703-694-8_15

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