Author: Hsin Yi Huang

0000000000485143

AUTHOR

Hsin Yi Huang

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Isolation of Zebrafish Gonads for RNA Isolation

2013

Piwi proteins and piRNAs are abundant in the gonads of various animal species. Gonads from different developmental stages provide us information regarding the function of piRNAs and the PIWI pathway during germline development. Here we describe methods for gonad and germ cell preparation from different developmental stages of zebrafish. We also describe how to use these gonads to purify and characterize piRNAs.

endocrine systemGonadurogenital systemPiwi-interacting RNABiologybiology.organism_classificationGermlineCell biologymedicine.anatomical_structuremedicineRNA extractionAnimal speciesZebrafishGerm cellFunction (biology)

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PEPhysical Sciences and Engineering
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