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RESEARCH PRODUCT
Study of elution behaviour with gradient voltage in CEC using methacrylate monolithic columns.
José Manuel Herrero-martínezJuan José Baeza-baezaErnesto F. Simó-alfonsoVirginia Bernabé-zafónsubject
Monolithic HPLC columnChemistryElutionClinical BiochemistryAnalytical chemistryTEST MixtureMethacrylateBiochemistryAnalytical Chemistrysymbols.namesakeCapillary ElectrochromatographysymbolsConstant voltageMethacrylatesConstant (mathematics)Gaussian network modelMathematicsVoltagedescription
A theoretical study on the retention behaviour and chromatographic performance of neutral solutes using a lauryl methacrylate-based monolithic column under voltage gradient mode in CEC was carried out. Through a flexible mathematical function based on a modified Gaussian model, the peak shape of compounds was firstly fitted under constant and gradient voltage. Using the peak shape parameters and retention time, the estimation of global chromatographic performance, efficiency and peak capacity under several voltage conditions was performed. The influence of voltage gradient on the separation efficiency is discussed and simple equations are presented to calculate retention and peak widths under voltage gradient conditions. A comparison in terms of chromatographic performance of a test mixture of neutral solutes under constant and gradient voltage modes was also carried out. The experiments carried out under gradient voltage showed better efficiencies (172,000 plates/m) and lower peak widths than those obtained under constant voltage (52,000 plates/m).
year | journal | country | edition | language |
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2010-03-24 | Electrophoresis |