6533b821fe1ef96bd127b839

RESEARCH PRODUCT

Additional file 4 of Intracellular osteopontin protects from autoimmunity-driven lymphoma development inhibiting TLR9-MYD88-STAT3 signaling

subject

description

Additional file 4: Supplementary Figure S1. Evaluation of autoimmunity in Faslpr/lpr and OPN-/-Faslpr/lpr mice. A. Quantification of OPN in sera from Faslpr/lpr mice at 2 (n=8) and 5 months of age (n=7) by ELISA. Sera from BALB/c and OPN-/- mice were tested as controls. Data are expressed as ng/ml and are a pool of 2 experiments (*, P<0.05; Ordinary one way ANOVA). B. Flow cytometry analysis showing the relative number of splenic autoimmune CD3+B220+ T cells in Faslpr/lpr (n=15) and OPN-/-Faslpr/lpr mice (n=18) and at about 5-6 months of age. The graph shows a pool of 3 different experiments (***, P<0.001; Student t test). C. Representative spleen photograph from BALB/c, OPN-/-, Faslpr/lpr and OPN-/-Faslpr/lpr mice. D. Representative H/E staining of spleen samples from 5 month-old Faslpr/lpr and OPN-/-Faslpr/lpr mice. Reactive lymphoid cells in OPN-competent animals and initial lymphomatous foci in OPN-deficient counterparts are shown by black arrows, respectively. Magnification 20x (left) and 40x (right). E. Flow cytometry analysis showing the relative number of peripheral blood autoimmune CD3+B220+ T cells in and Faslpr/lpr (n=6) and OPN-/-Faslpr/lpr mice (n=7) at about 5-6 months of age. The graph refers to one representative experiment (*, P<0.05; Student t test).